optical density od values  (Danaher Inc)

Journal: Vaccines

Article Title: Circulating Antibodies Against Common Cold Coronaviruses Do Not Interfere with Immune Responses to Primary or Booster SARS-CoV-2 mRNA Vaccines.

doi: 10.3390/vaccines13050547

Figure Lengend Snippet: Figure 3. This figure illustrates the optical density (OD) values representing IgG antibody (Ab) levels against conserved and specific antigens of seasonal common cold coronaviruses (CCCoVs) across seven time points: pre-vaccination (Pre), first vaccine dose (Vax1), second vaccine dose (Vax2), 6 months post-vaccination (6 m), 9 months post-vaccination (9 m), 12 months post-vaccination (12 m), and 15 months post-vaccination (15 m). Serum samples were diluted 1:100 for the detection of the IgG antibody against CCCoVs. The left column displays IgG Ab levels against alpha CCCoVs (A,C,E,G), while the right column represents beta CCCoVs (B,D,F,H). Alpha CCCoV-specific IgG Ab levels and beta CCCoV-specific IgG Ab levels were measured using S protein (RBD) and N protein peptide ELISAs. The graphs are subdivided into anti-S Ab levels (top two rows) and anti-N Ab levels (bottom two rows) for each virus. Additionally, the bottom-most row shows IgG Ab levels against conserved N peptides for alpha CCCoVs (I) and beta CCCoVs (J). The red dots indicate individual data points, with lines connecting mean OD values across time points; different letters indicate statistically significant differences. Different time points that share the same letter are not significantly different from each other, while the time points with different letters indicate significant differences (p < 0.05). Data are presented as mean ± SEM.

Article Snippet: The development of the plates followed previously described methods, and optical density (OD) values were measured at 650 nm using SoftMax Pro 7.1 software (Molecular Devices, LLC, San Jose, CA, USA).

Techniques: Virus

Journal: Vaccines

Article Title: Circulating Antibodies Against Common Cold Coronaviruses Do Not Interfere with Immune Responses to Primary or Booster SARS-CoV-2 mRNA Vaccines.

doi: 10.3390/vaccines13050547

Figure Lengend Snippet: Figure 4. This figure illustrates the optical density (OD) values representing IgA antibody (Ab) levels against conserved and specific antigens of seasonal common cold coronaviruses (CCCoVs) across seven time points: pre-vaccination (Pre), first vaccine dose (Vax1), second vaccine dose (Vax2), 6 months post-vaccination (6 m), 9 months post-vaccination (9 m), 12 months post-vaccination (12 m), and 15 months post-vaccination (15 m). Serum samples were diluted 1:100 for the detection of the IgG antibody against CCCoVs. The left column displays IgA Ab levels against alpha CCCoVs (A,C,E,G), while the right column represents beta CCCoVs (B,D,F,H). Alpha CCCoV-specific IgA Ab levels and beta CCCoV-specific IgA Ab levels were measured using S protein (RBD) and N protein peptide ELISAs. The graphs are subdivided into anti-S Ab levels (top two rows) and anti-N Ab levels (bottom two rows) for each virus. Additionally, the bottom-most row shows IgA Ab levels against conserved N peptides for alpha CCCoVs (I) and beta CCCoVs (J). The red dots indicate individual data points, with lines connecting mean OD values across time points; different letters indicate statistically significant differences. Different time points that share the same letter are not significantly different from each other, while the time points with different letters indicate significant differences (p < 0.05). Data are presented as mean ± SEM.

Article Snippet: The development of the plates followed previously described methods, and optical density (OD) values were measured at 650 nm using SoftMax Pro 7.1 software (Molecular Devices, LLC, San Jose, CA, USA).

Techniques: Virus

Journal: Vaccines

Article Title: Circulating Antibodies Against Common Cold Coronaviruses Do Not Interfere with Immune Responses to Primary or Booster SARS-CoV-2 mRNA Vaccines.

doi: 10.3390/vaccines13050547

Figure Lengend Snippet: Figure 5. This figure illustrates the optical density (OD) values representing IgM antibody (Ab) levels against conserved and specific antigens of seasonal common cold coronaviruses (CCCoVs) across seven time points: pre-vaccination (Pre), first vaccine dose (Vax1), second vaccine dose (Vax2), 6 months post-vaccination (6 m), 9 months post-vaccination (9 m), 12 months post-vaccination (12 m), and 15 months post-vaccination (15 m). Serum samples were diluted 1:100 for the detection of IgG antibody against CCCoVs. The left column displays IgM Ab levels against alpha CCCoVs (A,C,E,G), while the right column represents beta CCCoVs (B,D,F,H). Alpha CCCoV-specific IgM Ab levels and beta CCCoV-specific IgM Ab levels were measured using S protein (RBD) and N protein peptide ELISAs. The graphs are subdivided into anti-S Ab levels (top two rows) and anti-N Ab levels (bottom two rows) for each virus. Additionally, the bottom-most row shows IgM Ab levels against conserved N peptides for alpha CCCoVs (I) and beta CCCoVs (J). The red dots indicate individual data points, with lines connecting mean OD values across time points; different letters indicate statistically significant differences. Different time points that share the same letter are not significantly different from each other, while the time points with different letters indicate significant differences (p < 0.05). Data are presented as mean ± SEM.

Article Snippet: The development of the plates followed previously described methods, and optical density (OD) values were measured at 650 nm using SoftMax Pro 7.1 software (Molecular Devices, LLC, San Jose, CA, USA).

Techniques: Virus